Version 1
: Received: 30 January 2023 / Approved: 31 January 2023 / Online: 31 January 2023 (10:23:56 CET)
Version 2
: Received: 1 February 2023 / Approved: 2 February 2023 / Online: 2 February 2023 (11:52:20 CET)
Somodi, L.; Horváth, E.; Bárdos, H.; Baráth, B.; Pethő, D.; Katona, É.; Balla, J.; Mutch, N.J.; Muszbek, L. Cellular FXIII in Human Macrophage-Derived Foam Cells. Int. J. Mol. Sci.2023, 24, 4802.
Somodi, L.; Horváth, E.; Bárdos, H.; Baráth, B.; Pethő, D.; Katona, É.; Balla, J.; Mutch, N.J.; Muszbek, L. Cellular FXIII in Human Macrophage-Derived Foam Cells. Int. J. Mol. Sci. 2023, 24, 4802.
Somodi, L.; Horváth, E.; Bárdos, H.; Baráth, B.; Pethő, D.; Katona, É.; Balla, J.; Mutch, N.J.; Muszbek, L. Cellular FXIII in Human Macrophage-Derived Foam Cells. Int. J. Mol. Sci.2023, 24, 4802.
Somodi, L.; Horváth, E.; Bárdos, H.; Baráth, B.; Pethő, D.; Katona, É.; Balla, J.; Mutch, N.J.; Muszbek, L. Cellular FXIII in Human Macrophage-Derived Foam Cells. Int. J. Mol. Sci. 2023, 24, 4802.
Abstract
The potentially active A subunit of coagulation factor XIII (FXIII-A) is an intracellular transglutaminase expressed in various cell types including platelets and monocytes/macrophages. It is involved in stabilizing protein structures by cross-linking through Nε-(?-L-glutamyl)-L-lysyl iso-peptide bonds. Macrophages are major cellular constituents of the atherosclerotic plaque and are important in determining its structural/functional features. Two of their important functions are the accumulation of oxidized LDL in the lipid core, and by cross-linking structural proteins they may stabilize the plaque and protect the thrombi of atherogenic origin against fibrinolytic degradation. It is important to know whether these functions operate in parallel utilizing the same cellular compartments. First, we showed that monocyte-derived human macrophages significantly increase their FXIII-A content when up-taking oxidized LDL. This phenomenon is very likely independent of the process of transformation into foam cells, as the transformation of vascular smooth muscle cells into foam cells fails to result in the expression of FXIII-A. FXIII containing macrophage-like cells are abundant in the plaque and FXIII-A is also present in the extracellular core. Several cells co-stained for FXIII-A and for Oil Red O suggest that expression of FXIII-A and lipid up-take are common features of macrophages present in the atherosclerotic plaque.
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
2 February 2023
Commenter:
L�szl� Muszbek
Commenter's Conflict of Interests:
Author
Comment:
The manuscript was supplemented with the following changes additions: e-mail address of Nicola J. Mutch: [email protected] Institutional Review Board Statement: This study on tissue sections was conducted according to the principles of the Helsinki Declaration and was approved by the Institutional Review Board ofCounty Emergency Clinical Hospital ofTargu Mures, Romania(no. 29496/2019). Informed Consent Statement: Written informed consent was obtained from each patient involved in the study.
Commenter: L�szl� Muszbek
Commenter's Conflict of Interests: Author
e-mail address of Nicola J. Mutch: [email protected]
Institutional Review Board Statement: This study on tissue sections was conducted according to the principles of the Helsinki Declaration and was approved by the Institutional Review Board of County Emergency Clinical Hospital of Targu Mures, Romania (no. 29496/2019).
Informed Consent Statement: Written informed consent was obtained from each patient involved in the study.